The Cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the Plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
Catalog No
A7079
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81
Molecular Weight (Da)
69413
Gene Name
EZR
Protein Name
Ezrin
Human Gene ID
7430
Human Swiss Prot No.
P15311
Immunogen
The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Thr566. AA range:534-583
Specificity
Phospho-Ezrin (T566) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at T566.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated PMA 125ng/ml 30', using Ezrin (Phospho-Thr566) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ezrin (Phospho-Thr566) Antibody
