Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiProtein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]
Catalog No
A7100
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HDAC4; KIAA0288; Histone deacetylase 4; HD4
Molecular Weight (Da)
119070
Gene Name
HDAC4
Protein Name
Histone deacetylase 4
Human Gene ID
9759
Human Swiss Prot No.
P56524
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC4 around the phosphorylation site of Ser632. AA range:598-647
Specificity
Phospho-HDAC4 (S632) Polyclonal Antibody detects endogenous levels of HDAC4 protein only when phosphorylated at S632.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1hour and Jurkat cells treated with etoposide 25uM 24hours, using HDAC4 (Phospho-Ser632) Antibody. The lane on the right is blocked with the phospho peptide.