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Primary Antibodies
IκB-α (Phospho-Ser32/S36) Antibody
IκB-α (Phospho-Ser32/S36) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A7124
Reactivity:Human,Mouse,Rat,Monkey
Applications:WB,IHC,IF,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
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IκB-α (Phospho-Ser32/S36) Antibody

Immunofluorescence analysis of rat-spleen tissue. 1,IκB-α (phospho Ser32/S36) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Product Details
Product Name
IκB-α (Phospho-Ser32/S36) Antibody
Description
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011],
Catalog No
A7124
Reactivity
Human, Mouse, Rat, Monkey
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
WB, IHC, IF, ELISA
Dilution
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Molecular Weight (Da or kDa)
Gene Name
NFKBIA IKBA MAD3 NFKBI
Protein Name
NF-kappa-B inhibitor alpha
Human Gene ID
4792
Human Swiss Prot No.
P25963
Immunogen
The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Ser32/Ser36. AA range:15-64
Specificity
Phospho-IκB-α (S32/S36) Polyclonal Antibody detects endogenous levels of IκB-α protein only when phosphorylated at S32/S36.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (10)
MAST3 modulates the inflammatory response and proliferation of fibroblast-like synoviocytes in rheumatoid arthritis. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2019 Dec;77:105900 WB Rat 1:500 Synovial tissues FLSs

Author: Available in link provided

Date of Publication : Available in link provided

NLRC5 promotes cell proliferation via regulating the NF-κB signaling pathway in Rheumatoid arthritis. MOLECULAR IMMUNOLOGY Mol Immunol. 2017 Nov;91:24 WB Rat 1:500 Synovial tissues FLS cell

Author: Available in link provided

Date of Publication : Available in link provided

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2015 Sep;28:695 WB Mouse 1:1000 uterine tissues

Author: Available in link provided

Date of Publication : Available in link provided

PSTPIP2 Inhibits the Inflammatory Response and Proliferation of Fibroblast-Like Synoviocytes in vitro. Frontiers in Pharmacology Front Pharmacol. 2018 Dec;9:1432 WB Rat 1:1000 Synovial tissues Fibroblast-like synoviocytes (FLSs)

Author: Available in link provided

Date of Publication : Available in link provided

Activation of NLRP3 inflammasome up-regulates TREM-1 expression in murine macrophages via HMGB1 and IL-18. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2020 Dec;89:107045 WB Mouse 1:1000 Lung Macrophage

Author: Available in link provided

Date of Publication : Available in link provided

miR‐377‐3p drives malignancy characteristics via upregulating GSK‐3β expression and activating NF‐κB pathway in hCRC cells. JOURNAL OF CELLULAR BIOCHEMISTRY J Cell Biochem. 2018 Feb;119(2):2124-2134 WB Human HCT116 cell,SW480 cell

Author: Available in link provided

Date of Publication : Available in link provided

The adhesion protein of Mycoplasma genitalium inhibits urethral epithelial cell apoptosis through CypA-CD147 activating PI3K/ Akt/NF-κB pathway APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Yanhua Zeng WB Human

Author: Available in link provided

Date of Publication : Available in link provided

Surface functionalization-dependent inflammatory potential of polystyrene nanoplastics through the activation of MAPK/ NF-κB signaling pathways in macrophage Raw 264.7 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jian Xu WB Mouse RAW 264.7 cells

Author: Available in link provided

Date of Publication : Available in link provided

Effects of Aire on perforin expression in BMDCs via TLR7/8 and its therapeutic effect on type 1 diabetes INTERNATIONAL IMMUNOPHARMACOLOGY Wei Yang WB Mouse bone marrow-derived dendritic cells (BMDCs)

Author: Available in link provided

Date of Publication : Available in link provided

Polystyrene nanoplastics induce intestinal and hepatic inflammation through activation of NF-κB/NLRP3 pathways and related gut-liver axis in mice SCIENCE OF THE TOTAL ENVIRONMENT Xuanwei Chen WB Mouse 1:1000 intestinal tissue,liver tissue

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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