There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog
Catalog No
A7140
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
LIMK1; LIMK; LIM domain kinase 1; LIMK-1
Molecular Weight (Da)
72232
Gene Name
LIMK1
Protein Name
LIM domain kinase 1
Human Gene ID
3984
Human Swiss Prot No.
P53667
Immunogen
The antiserum was produced against synthesized peptide derived from human LIMK1 around the phosphorylation site of Thr508. AA range:471-520
Specificity
Phospho-LIMK-1 (T508) Polyclonal Antibody detects endogenous levels of LIMK-1 protein only when phosphorylated at T508.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using LIMK1 (Phospho-Thr508) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using LIMK1 (Phospho-Thr508) Antibody
Gao, Ting-Ting, et al. "LIMK1/2 in the mPFC plays a role in chronic stress-induced depressive-like effects in mice." International Journal of Neuropsychopharmacology 23.12 (2020): 821-836.
