The protein encoded by this gene is a substrate for protein kinase C. It is localized to the Plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the Plasma membrane, leading to its presence in the Cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]
Catalog No
A7143
Reactivity
Human, Mouse, Rat
Applications
IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
MARCKS; MACS; PRKCSL; Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate; 80 kDa protein, light chain; 80K-L protein; PKCSL
Molecular Weight (Da)
31555
Gene Name
MARCKS
Protein Name
Myristoylated alanine-rich C-kinase substrate
Human Gene ID
4082
Human Swiss Prot No.
P29966
Immunogen
The antiserum was produced against synthesized peptide derived from human MARCKS around the phosphorylation site of Ser163. AA range:136-185
Specificity
Phospho-MARCKS (S163) Polyclonal Antibody detects endogenous levels of MARCKS protein only when phosphorylated at S163.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human brain, using MARCKS (Phospho-Ser163) Antibody. The picture on the right is blocked with the phospho peptide.
