The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
Catalog No
A7158
Reactivity
Human, Mouse, Rat
Applications
IHC-p, IF(paraffin section), IP, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
Molecular Weight (Da)
48804
Gene Name
MYC
Protein Name
Myc proto-oncogene protein
Human Gene ID
4609
Human Swiss Prot No.
P01106
Immunogen
The antiserum was produced against synthesized peptide derived from human Myc around the phosphorylation site of Thr358. AA range:325-374
Specificity
Phospho-c-Myc (T358) Polyclonal Antibody detects endogenous levels of c-Myc protein only when phosphorylated at T358.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Thr358) Antibody. The picture on the right is blocked with the phospho peptide.
