Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]
Catalog No
A7160
Reactivity
Human, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NBN; NBS; NBS1; P95; Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1
Molecular Weight (Da)
84959
Gene Name
NBN
Protein Name
Nibrin
Human Gene ID
4683
Human Swiss Prot No.
O60934
Immunogen
The antiserum was produced against synthesized peptide derived from human p95/NBS1 around the phosphorylation site of Ser343. AA range:310-359
Specificity
Phospho-Nibrin (S343) Polyclonal Antibody detects endogenous levels of Nibrin protein only when phosphorylated at S343.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells, using p95/NBS1 (Phospho-Ser343) Antibody. The lane on the left is blocked with the phospho peptide.
