nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Catalog No
A7164
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IP, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52 around the phosphorylation site of Ser865. AA range:833-882
Specificity
Phospho-NFκB-p100 (S865) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S865.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from ovary cancer, using NF-kappaB p100/p52 (Phospho-Ser865) Antibody. The lane on the left is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser865) Antibody. The picture on the right is blocked with the phospho peptide.
