The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the Extracellular matrix into the Cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Catalog No
A8079
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
AXL; UFO; Tyrosine-protein kinase receptor UFO; AXL oncogene
Molecular Weight (Da)
97437
Gene Name
AXL
Protein Name
Tyrosine-protein kinase receptor UFO
Human Gene ID
558
Human Swiss Prot No.
P30530
Immunogen
The antiserum was produced against synthesized peptide derived from human AXL around the phosphorylation site of Tyr691. AA range:657-706
Specificity
Phospho-Axl (Y691) Polyclonal Antibody detects endogenous levels of Axl protein only when phosphorylated at Y691.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 15', using AXL (Phospho-Tyr691) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using AXL (Phospho-Tyr691) Antibody. The picture on the right is blocked with the phospho peptide.
