The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Catalog No
A8128
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
MAK; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase
Molecular Weight (Da)
70581
Gene Name
MAK
Protein Name
Serine/threonine-protein kinase MAK
Human Gene ID
4117
Human Swiss Prot No.
P20794
Immunogen
The antiserum was produced against synthesized peptide derived from human MAK around the phosphorylation site of Tyr159. AA range:126-175
Specificity
Phospho-MAK (Y159) Polyclonal Antibody detects endogenous levels of MAK protein only when phosphorylated at Y159.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using MAK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.