This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Catalog No
A8151
Reactivity
Human, Rat, Mouse
Applications
IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PKMYT1; MYT1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase
Molecular Weight (Da)
54521
Gene Name
PKMYT1
Protein Name
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Human Gene ID
9088
Human Swiss Prot No.
Q99640
Immunogen
The antiserum was produced against synthesized peptide derived from human MYT1 around the phosphorylation site of Ser83. AA range:49-98
Specificity
Phospho-Myt 1 (S83) Polyclonal Antibody detects endogenous levels of Myt 1 protein only when phosphorylated at S83.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYT1 (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MYT1 (Phospho-Ser83) Antibody
