ribosomal protein S6 kinase B2(RPS6KB2) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015]
Catalog No
A8157
Reactivity
Human, Mouse
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase beta around the phosphorylation site of Ser423. AA range:389-438
Specificity
Phospho-p70 S6 kinase β (S423) Polyclonal Antibody detects endogenous levels of p70 S6 kinase β protein only when phosphorylated at S423.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody. The lane on the right is blocked with the phospho peptide.