This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive Cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Catalog No
A8203
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
IRF3; Interferon regulatory factor 3; IRF-3
Molecular Weight (Da)
47219
Gene Name
IRF3
Protein Name
Interferon regulatory factor 3
Human Gene ID
3661
Human Swiss Prot No.
Q14653
Immunogen
The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser386. AA range:352-401
Specificity
Phospho-IRF-3 (S386) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S386.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser386) Antibody. The lane on the right is blocked with the phospho peptide.