This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via Methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Catalog No
A8262
Reactivity
Human, Rat, Mouse
Applications
IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CBX5; HP1A; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha
Molecular Weight (Da)
22225
Gene Name
CBX5
Protein Name
Chromobox protein homolog 5
Human Gene ID
23468
Human Swiss Prot No.
P45973
Immunogen
The antiserum was produced against synthesized peptide derived from human HP1 alpha around the phosphorylation site of Ser92. AA range:58-107
Specificity
Phospho-HP1α (S92) Polyclonal Antibody detects endogenous levels of HP1α protein only when phosphorylated at S92.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HP1 alpha (Phospho-Ser92) Antibody. The picture on the right is blocked with the phospho peptide.
