This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Catalog No
A8307
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
Molecular Weight (Da)
37575
Gene Name
CEBPA
Protein Name
CCAAT/enhancer-binding protein alpha
Human Gene ID
1050
Human Swiss Prot No.
P49715
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human C/EBP α (phospho Thr230)
Specificity
Phospho-C/EBP α (T230) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at T230.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
