This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Catalog No
A8324
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DPYSL2; CRMP2; ULIP2; Dihydropyrimidinase-related protein 2; DRP-2; Collapsin response mediator protein 2; CRMP-2; N2A3; Unc-33-like phosphoprotein 2; ULIP-2
Molecular Weight (Da)
62294
Gene Name
DPYSL2
Protein Name
Dihydropyrimidinase-related protein 2
Human Gene ID
1808
Human Swiss Prot No.
Q16555
Immunogen
The antiserum was produced against synthesized peptide derived from human CRMP-2 around the phosphorylation site of Thr509. AA range:475-524
Specificity
Phospho-CRMP-2 (T509) Polyclonal Antibody detects endogenous levels of CRMP-2 protein only when phosphorylated at T509.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HT29 cells treated with heat shock, using CRMP-2 (Phospho-Thr509) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using CRMP-2 (Phospho-Thr509) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).