This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group Protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Catalog No
A8325
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CTBP1; CTBP; C-terminal-binding protein 1; CtBP1
Molecular Weight (Da)
47535
Gene Name
CTBP1
Protein Name
C-terminal-binding protein 1
Human Gene ID
1487
Human Swiss Prot No.
Q13363
Immunogen
The antiserum was produced against synthesized peptide derived from human CtBP1 around the phosphorylation site of Ser422. AA range:388-437
Specificity
Phospho-CtBP1 (S422) Polyclonal Antibody detects endogenous levels of CtBP1 protein only when phosphorylated at S422.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30' and Jurkat cells treated with UV 15', using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using CtBP1 (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.