The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencep
Catalog No
A8336
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CCND2; G1/S-specific cyclin-D2
Molecular Weight (Da)
33067
Gene Name
CCND2
Protein Name
G1/S-specific cyclin-D2
Human Gene ID
894
Human Swiss Prot No.
P30279
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin D2 around the phosphorylation site of Thr280. AA range:240-289
Specificity
Phospho-Cyclin D2 (T280) Polyclonal Antibody detects endogenous levels of Cyclin D2 protein only when phosphorylated at T280.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COLO205 cells treated with EGF 200ng/ml 30', using Cyclin D2 (Phospho-Thr280) Antibody. The lane on the right is blocked with the phospho peptide.
