This gene encodes a member of the doublecortin family. The protein encoded by this gene is a Cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain&quo
Catalog No
A8337
Reactivity
Human, Mouse, Rat
Applications
IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DCX; DBCN; LISX; Neuronal migration protein doublecortin; Doublin; Lissencephalin-X; Lis-X
Molecular Weight (Da)
44925
Gene Name
DCX
Protein Name
Neuronal migration protein doublecortin
Human Gene ID
1641
Human Swiss Prot No.
O43602
Immunogen
The antiserum was produced against synthesized peptide derived from human Doublecortin around the phosphorylation site of Ser376. AA range:330-365
Specificity
Phospho-Doublecortin (S339) Polyclonal Antibody detects endogenous levels of Doublecortin protein only when phosphorylated at S339.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Doublecortin (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Doublecortin (Phospho-Ser376) Antibody
