The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]
Catalog No
A8341
Reactivity
Human, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EPB41; E41P; Protein 4.1; P4.1; 4.1R; Band 4.1; EPB4.1
Molecular Weight (Da)
97017
Gene Name
EPB41
Protein Name
Protein 4.1
Human Gene ID
2035
Human Swiss Prot No.
P11171
Immunogen
The antiserum was produced against synthesized peptide derived from human EPB41 around the phosphorylation site of Tyr660/418. AA range:626-675
Specificity
Phospho-4.1R (Y660) Polyclonal Antibody detects endogenous levels of 4.1R protein only when phosphorylated at Y660.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using EPB41 (Phospho-Tyr660/418) Antibody. The lane on the right is blocked with the phospho peptide.