This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Catalog No
A8363
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2
Molecular Weight (Da)
45568
Gene Name
MAPKAPK2
Protein Name
MAP kinase-activated protein kinase 2
Human Gene ID
9261
Human Swiss Prot No.
P49137
Immunogen
The antiserum was produced against synthesized peptide derived from human MAPKAPK2 around the phosphorylation site of Ser272. AA range:238-287
Specificity
Phospho-MAPKAPK-2 (S272) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at S272.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Ser272) Antibody. The picture on the right is blocked with the phospho peptide.