RAD50 double strand break repair protein(RAD50) Homo sapiens The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The Protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
Catalog No
A8409
Reactivity
Human, Rat, Mouse
Applications
WB
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DNA repair protein RAD50 (hRAD50) (EC 3.6.-.-)
Molecular Weight (Da)
Gene Name
RAD50
Protein Name
Rad50 (Ser635)
Human Gene ID
10111
Human Swiss Prot No.
Q92878
Immunogen
Synthesized phosho peptide around human Rad50 (Ser635)
Specificity
This antibody detects endogenous levels of Human Rad50 (phospho-Ser635)
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
