docking protein 2(DOK2) Homo sapiens The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]
Catalog No
A8467
Reactivity
Human, Mouse
Applications
IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DOK2; Docking protein 2; Downstream of tyrosine kinase 2; p56(dok-2)
Molecular Weight (Da)
45379
Gene Name
DOK2
Protein Name
Docking protein 2
Human Gene ID
9046
Human Swiss Prot No.
O60496
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Dok-2 (phospho Tyr345)
Specificity
Phospho-Dok-2 (Y345) Polyclonal Antibody detects endogenous levels of Dok-2 protein only when phosphorylated at Y345.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
