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Primary Antibodies
MYLK (Phospho-Tyr464) Antibody
MYLK (Phospho-Tyr464) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A8524
Reactivity:Human,Rat,Mouse
Applications:IHC,IF,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
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MYLK (Phospho-Tyr464) Antibody

Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200

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Product Details
Product Name
MYLK (Phospho-Tyr464) Antibody
Description
myosin light chain kinase(MYLK) Homo sapiens This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity.This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been
Catalog No
A8524
Reactivity
Human, Rat, Mouse
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
IHC, IF, ELISA
Dilution
IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
MYLK; MLCK; MLCK1; MYLK1; Myosin light chain kinase; smooth muscle; MLCK; smMLCK; Kinase-related protein; KRP; Telokin
Molecular Weight (Da or kDa)
211kD
Gene Name
MYLK
Protein Name
Myosin light chain kinase smooth muscle
Human Gene ID
4638
Human Swiss Prot No.
Q15746
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human MYLK (phospho Tyr464)
Specificity
Phospho-MYLK (Y464) Polyclonal Antibody detects endogenous levels of MYLK protein only when phosphorylated at Y464.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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