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SIRT1 (Phospho-Ser549) Antibody
SIRT1 (Phospho-Ser549) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A9666
Reactivity:Human,Mouse
Applications:WB,ELISA,IHC
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

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SIRT1 (Phospho-Ser549) Antibody

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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Product Details
Product Name
SIRT1 (Phospho-Ser549) Antibody
Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],
Catalog No
A9666
Reactivity
Human, Mouse
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
WB, ELISA, IHC
Dilution
WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
NAD-dependent protein deacetylase sirtuin-1 (hSIRT1; EC 3.5.1.-; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2) [Cleaved into: SirtT1 75 kDa fragment (75SirT1)]
Molecular Weight (Da or kDa)
Gene Name
SIRT1 SIR2L1
Protein Name
SIRT1 (phospho-Ser549)
Human Gene ID
23411
Human Swiss Prot No.
Q96EB6
Immunogen
Synthesized peptide derived from human SIRT1 (phospho-Ser549)
Specificity
This antibody detects endogenous levels of Human,Mouse SIRT1 (phospho-Ser549)
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (1)
Generation and characterization of a site-specific antibody for SIRT1 O-GlcNAcylated at serine 549. GLYCOBIOLOGY Glycobiology. 2018 Jul;28(7):482-487 WB,ELISA Human,Mouse liver tissues HEK 293 T cell,NCI-H1299 cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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