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> Products > Primary Antibodies > Vimentin Antibody

Vimentin Antibody

Overview Description
Datasheet SDS
   Structural Proteins   
Catalog No : B1242
  • Reactivity
    Human,Mouse,Rat
  • Applications
    WB,IHC-p,IF/ICC,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
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Details
  • Product Name
    Vimentin Antibody
  • Description
    This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the Cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
  • Catalog No
    B1242
  • Reactivity
    Human, Mouse, Rat
  • Applications
    WB, IHC-p, IF/ICC, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    VIM; Vimentin
  • Molecular Weight (Da)
    53652
  • Gene Name
    VIM
  • Protein Name
    Vimentin
  • Human Gene ID
    7431
  • Human Swiss Prot No.
    P08670
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:56-105
  • Specificity
    Vimentin Polyclonal Antibody detects endogenous levels of Vimentin protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using Vimentin Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of various cells using Vimentin Polyclonal Antibody. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Application Data
Immunofluorescence analysis of Hela cell. 1,Vimentin Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Application Data
Wu, Yan, et al. "TrAmplification of Human Dental Follicle Cells by piggyBac Transposon-Mediated Reversible Immortalization System." PloS one 10.7 (2015): e0130937.
Application Data
Zou, Guoying, et al. "Inhibin B suppresses anoikis resistance and migration through the transforming growth factor‐β signaling pathway in nasopharyngeal carcinoma." Cancer science 109.11 (2018): 3416.
Publications

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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