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Primary Antibodies
Histone H2A.X Antibody
Histone H2A.X Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: B7106
Reactivity:Human,Mouse,Rat
Applications:WB,IHC,IF,CoIP,ELISA
Modification:Unmodified/Total
Source:Polyclonal,Rabbit,IgG
Storage and Stability:Store at -20°C/1 year

$205
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Histone H2A.X Antibody

Immunofluorescence analysis of rat-lung tissue. 1,Histone H2A.X Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Product Details
Product Name
Histone H2A.X Antibody
Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],
Catalog No
B7106
Reactivity
Human, Mouse, Rat
Source
Polyclonal, Rabbit,IgG
Modification
Unmodified/Total
Applications
WB, IHC, IF, CoIP, ELISA
Dilution
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. IP 1:200-500,ELISA: 1:10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
H2AFX; H2AX; Histone H2A.x; H2a/x
Molecular Weight (Da or kDa)
Gene Name
H2AFX
Protein Name
Histone H2A.x
Human Gene ID
3014
Human Swiss Prot No.
P16104
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2A.X. AA range:94-143
Specificity
Histone H2A.X Polyclonal Antibody detects endogenous levels of Histone H2A.X protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (3)
A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination CoIP Human /HeLa cell，U2OS cell

Author: Available in link provided

Date of Publication : Available in link provided

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics Cancer Lett. 2022 Apr;530:29. WB,IP Human

Author: Available in link provided

Date of Publication : Available in link provided

Marine bromophenol bis (2, 3-dibromo-4, 5-dihydroxybenzyl) ether, represses angiogenesis in HUVEC cells and in zebrafish embryos via inhibiting the VEGF signal systems." Biomedicine & Pharmacotherapy 75 (2015): 58-66.

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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