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> Products > Primary Antibodies > FN1 Antibody

FN1 Antibody

Overview Description
Datasheet SDS
   Cell Adhesion Proteins   
Catalog No : C0195
  • Reactivity
    Human,Mouse,Rat
  • Applications
    WB,IHC-p,IF(paraffin section),ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
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Details
  • Product Name
    FN1 Antibody
  • Description
    This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in Extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
  • Catalog No
    C0195
  • Reactivity
    Human, Mouse, Rat
  • Applications
    WB, IHC-p, IF(paraffin section), ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG
  • Molecular Weight (Da)
    262607
  • Gene Name
    FN1
  • Protein Name
    Fibronectin
  • Human Gene ID
    2335
  • Human Swiss Prot No.
    P02751
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Fibronectin 1. AA range:2337-2386
  • Specificity
    FN1 Polyclonal Antibody detects endogenous levels of FN1 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from 1) 3T3, 2) KB,3) K562,4) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:N1292) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Application Data
Western blot analysis of lysates from HeLa cells, using Fibronectin 1 Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Fibronectin 1 Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Western blot analysis of 3T3 KB K562 293T lysis using FN1 antibody. Antibody was diluted at 1:2000
Application Data
Western Blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1:2000
Application Data
Western Blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1:2000
Application Data
Western blot analysis of lysates from 1) 3T3, 2) KB,3) K562,4) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) (cat:N1292) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Publications

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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