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Primary Antibodies
MMP-9 Antibody
MMP-9 Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: C0275
Reactivity:Human,Mouse,Rat
Applications:WB,IHC,IF,ELISA
Modification:Unmodified/Total
Source:Polyclonal,Rabbit,IgG
Storage and Stability:Store at -20°C/1 year

$205
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MMP-9 Antibody

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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Product Details
Product Name
MMP-9 Antibody
Description
matrix metallopeptidase 9(MMP9) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008],
Catalog No
C0275
Reactivity
Human, Mouse, Rat
Source
Polyclonal, Rabbit,IgG
Modification
Unmodified/Total
Applications
WB, IHC, IF, ELISA
Dilution
WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Molecular Weight (Da or kDa)
Gene Name
MMP9
Protein Name
Matrix metalloproteinase-9
Human Gene ID
4318
Human Swiss Prot No.
P14780
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP-9. AA range:651-700
Specificity
MMP-9 Polyclonal Antibody detects endogenous levels of MMP-9 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (35)
Methyl-CpG-binding domain 3 inhibits epithelial–mesenchymal transition in pancreatic cancer cells via TGF- β /Smad signalling. BRITISH JOURNAL OF CANCER 2016 Nov 29 WB Human SW1990 cell, PaTu8988 cell, PANC1 cell

Author: Available in link provided

Date of Publication : Available in link provided

Nigericin Exerts Anticancer Effects on Human Colorectal Cancer Cells by Inhibiting Wnt/β-catenin Signaling Pathway. MOLECULAR CANCER THERAPEUTICS Mol Cancer Ther. 2018 May;17(5):952-965 WB,IF Human 1:300 SW620 cell，KM12 cell

Author: Available in link provided

Date of Publication : Available in link provided

LncRNA UCA1 promotes migration and invasion in pancreatic cancer cells via the Hippo pathway. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 2018 Mar 03 WB Human BxPC-3 cell, SW1990 cell,PaTu8988 cell, PANC-1 cell

Author: Available in link provided

Date of Publication : Available in link provided

MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget Oncotarget. 2016 Mar 22; 7(12): 14476–14485 WB Human BxPC3 cell, PANC1 cell, SW1990 cell,PaTu8988 cell

Author: Available in link provided

Date of Publication : Available in link provided

CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2020 Jan;24(1):618-631 WB Human 1:1000 NCI-H460 cell, A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

MiR‐496 promotes migration and epithelial‐mesenchymal transition by targeting RASSF6 in colorectal cancer. JOURNAL OF CELLULAR PHYSIOLOGY J Cell Physiol. 2020 Feb;235(2):1469-1479 WB Human 1:1000 LoVo cell, SW480 cell

Author: Available in link provided

Date of Publication : Available in link provided

Inhibin B suppresses anoikis resistance and migration through the transforming growth factor‐β signaling pathway in nasopharyngeal carcinoma. CANCER SCIENCE Cancer Sci. 2018 Nov;109(11):3416-3427 WB Human HNE1 cell， 5-8F cell

Author: Available in link provided

Date of Publication : Available in link provided

Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway. BIOLOGICAL CHEMISTRY Biol Chem. 2021 Jun;402(7):805-813 WB Human 1 : 1000 SW480 cell，LoVo cell

Author: Available in link provided

Date of Publication : Available in link provided

Phosphoglycerate mutase 1 knockdown inhibits prostate cancer cell growth, migration, and invasion. ASIAN JOURNAL OF ANDROLOGY Asian J Androl. 2018 Mar-Apr; 20(2): 178–183 WB Human 1:500 PC-3 cell,22Rv1 cell,DU145 cell, LNCap cell

Author: Available in link provided

Date of Publication : Available in link provided

Endothelin-1 Activates the Notch Signaling Pathway and Promotes Tumorigenesis in Giant Cell Tumor of the Spine. SPINE Spine. 2019 Sep;44(17):E1000-E1009 WB Human 1:500 giant cell tumor (GCT) tissue

Author: Available in link provided

Date of Publication : Available in link provided

Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway. ONCOLOGY REPORTS 2016 Jul 28 WB Human U251 cell, A172 cell

Author: Available in link provided

Date of Publication : Available in link provided

Uridine-cytidine kinase 2 promotes metastasis of hepatocellular carcinoma cells via the Stat3 pathway. Cancer Management and Research Cancer Manag Res. 2018; 10: 6339–6355 WB Human 1:1000 Bel-7402 cell，Sk-hep-1 cell

Author: Available in link provided

Date of Publication : Available in link provided

Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2. CELLULAR ONCOLOGY Cell Oncol. 2021 Feb;44(1):93-107 WB Human 1:800 HeLa cell,SiHa cell

Author: Available in link provided

Date of Publication : Available in link provided

Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination. CELLULAR SIGNALLING Cell Signal. 2022 Mar;91:110230 WB Human 1:2000 HMrSV5 cell

Author: Available in link provided

Date of Publication : Available in link provided

miR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1. ONCOLOGY REPORTS Oncol Rep. 2015 Mar;33(3):1291-1299 WB Human Saos-2 cell, MG63 cell

Author: Available in link provided

Date of Publication : Available in link provided

Low-dose decitabine induces MAGE-A expression and inhibits invasion via suppression of NF-κB2 and MMP2 in Eca109 cells. BIOMEDICINE & PHARMACOTHERAPY 2014 Jul 18 WB Human 1:500 Eca109 cell

Author: Available in link provided

Date of Publication : Available in link provided

Protein arginine methyltransferase 1 coordinates the epithelial-mesenchymal transition/proliferation dichotomy in gastric cancer cells. EXPERIMENTAL CELL RESEARCH 2017 Oct 31 WB Human BGC823 cell, SGC7901 cell,AGS cell, MGC803 cell

Author: Available in link provided

Date of Publication : Available in link provided

YTH domain family 2 orchestrates epithelial-mesenchymal transition/proliferation dichotomy in pancreatic cancer cells. CELL CYCLE Cell Cycle. 2017;16(23):2259-2271 WB Human SW1990 cell, BxPC3 cell

Author: Available in link provided

Date of Publication : Available in link provided

Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells. American Journal of Translational Research Am J Transl Res. 2015; 7(9): 1660–1674 WB Human 1:1000 MDA-MB-231 cells,HS-5 cell

Author: Available in link provided

Date of Publication : Available in link provided

Long non‑coding RNA PVT1 promotes epithelial‑mesenchymal transition via the TGF‑β/Smad pathway in pancreatic cancer cells. ONCOLOGY REPORTS 2018 May 24 WB Human PaTu8988 cell, BxPc-3 cell

Author: Available in link provided

Date of Publication : Available in link provided

CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer. OncoTargets and Therapy Oncotargets Ther. 2016; 9: 7285–7295 WB Human PaTu8988 cell, SW1990 cell

Author: Available in link provided

Date of Publication : Available in link provided

Emodin suppresses the migration and invasion of melanoma cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN 2021 Mar 17 WB Mouse,Human 1 : 500 B16F10 Melanoma Cells， A375 Melanoma Cells

Author: Available in link provided

Date of Publication : Available in link provided

Paeonol exerts potential activities to inhibit the growth, migration and invasion of human gastric cancer BGC823 cells via downregulating MMP‑2 and MMP‑9. Molecular Medicine Reports Mol Med Rep. 2017 Nov;16(5):7513-7519 WB Human 1:1000 BGC823 cell

Author: Available in link provided

Date of Publication : Available in link provided

Zhang, Hong, et al. "Therapeutic potential of bixin in PM2. 5 particles-induced lung injury in an Nrf2-dependent manner." Free Radical Biology and Medicine 126 (2018): 166-176.

Author: Available in link provided

Date of Publication : Available in link provided

Fang, Zhiqing, et al. "Nitidine chloride inhibits renal cancer cell metastasis via suppressing AKT signaling pathway." Food and chemical toxicology 60 (2013): 246-251.

Author: Available in link provided

Date of Publication : Available in link provided

Integrated transcriptome and proteome revealed that the declined expression of cell cycle-related genes associated with follicular atresia in geese BMC GENOMICS Xingyong Chen WB Geese follicles

Author: Available in link provided

Date of Publication : Available in link provided

HMGB1 released from dead tumor cells after insufficient radiofrequency ablation promotes progression of HCC residual tumor via ERK1/2 pathway INTERNATIONAL JOURNAL OF HYPERTHERMIA Baoming Luo WB Human HepG2 cell

Author: Available in link provided

Date of Publication : Available in link provided

LncRNA FAM83A-AS1 promotes epithelial–mesenchymal transition of pancreatic cancer cells via Hippo pathway. Min Xu WB Human BxPC-3 cell,PANC-1 cell,PANC-1 cell, PaTu8988 cell

Author: Available in link provided

Date of Publication : Available in link provided

Bufalin inhibits the proliferation of lung cancer cells by suppressing Hippo-YAP pathway. Yongchun Yu WB Human A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

Rucaparib inhibits lung adenocarcinoma cell proliferation and migration via the SHCBP1/CDK1 pathway. FEBS Journal Rong Zhang WB Human 1 : 1000 A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway. Frontiers in Pharmacology Pengfei Yi WB Mouse lung tissue

Author: Available in link provided

Date of Publication : Available in link provided

MBD3 promotes epithelial-mesenchymal transition in gastric cancer cells by upregulating ACTG1 via the PI3K/AKT pathway BIOLOGICAL PROCEDURES ONLINE Wang Huizhi WB Human MGC-803 cell,HGC-27 cell

Author: Available in link provided

Date of Publication : Available in link provided

Unraveling the immune landscape and therapeutic biomarker PMEPA1 for oxaliplatin resistance in colorectal cancer: A comprehensive approach BIOCHEMICAL PHARMACOLOGY Zhengguang Zhang WB Human 1:1000 HCT116 cell

Author: Available in link provided

Date of Publication : Available in link provided

Modulation of epithelial-mesenchymal transition by gemcitabine: Targeting ionizing radiation-induced cellular senescence in lung cancer cell BIOCHEMICAL PHARMACOLOGY Heng Zhou WB,IHC Mouse,Human 1:200 Lewis cell-Xenograft A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

ATP11A Promotes Epithelial-mesenchymal Transition in Gastric Cancer Cells via the Hippo Pathway Journal of Cancer Wang Zhihua WB Human BGC-823 cell,MGC-803 cell,HGC-27 cell,SGC-7901 cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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