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Primary Antibodies
α-SMA Antibody
α-SMA Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: C47432
Reactivity:Human,Mouse,Rat
Applications:WB,IHC-p,IF(paraffin section),ELISA
Modification:Unmodified/Total
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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α-SMA Antibody

Western blot analysis of lysates from 1) Jurkat, 2) K562, 3)HuvEC cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. （Red） Tubulin β Monoclonal Antibody(5G3) (cat:N1294) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.

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Product Details
Product Name
α-SMA Antibody
Description
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Catalog No
C47432
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Molecular Weight (Da)
42009
Gene Name
ACTA2
Protein Name
Actin aortic smooth muscle
Human Gene ID
59
Human Swiss Prot No.
P62736
Immunogen
Synthesized peptide derived from the N-terminal region of human α-SMA.
Specificity
α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
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Publications (8)
SIRT6 inhibits endothelial-to-mesenchymal transition through attenuating the vascular endothelial inflammatory response

Author: Lifang Chen, Guan Wang, Jianyu He, Xin Yang, Zihan Zheng, Ying Deng, Yizhen Liu, Danli Chen, Rong Lin, Weirong Wang

Date of Publication : 2021-10-16

HDAC3 inhibitor suppresses endothelial-to-mesenchymal transition via modulating inflammatory response in atherosclerosis

Author: Lifang Chen, Chenxu Shang, Bo Wang, Guan Wang, Zhen Jin, Feng Yao, Zejun Yue, Liang Bai, Rong Wang, Sihai Zhao, Enqi Liu, Weirong Wang

Date of Publication : 2021-07-30

Ginsenoside Rg2 alleviates myocardial fibrosis by regulating TGF-β1/Smad signalling pathway

Author: Quanwei Wang , Wenwen Fu , Xiaofeng Yu , Huali Xu , Dayun Sui , Yeling Wang

Date of Publication : 2021-02-03

Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities

Author: Zhenyu Guo, Chentao Luo, Ting Zhu, Li Li, Wan Zhang

Date of Publication : 2020-04-28

Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-κB Pathway In Vitro

Author: Chunhua Wang, Hong Luo, Yini Xu, Ling Tao, Churui Chang, Xiangchun Shen

Date of Publication : 2018-10-26

Resveratrol inhibits monocrotaline-induced pulmonary arterial remodeling by suppression of SphK1-mediated NF-κB activation

Author: Wenhua Shi, Cui Zhai, Wei Feng, Jian Wang, Yanting Zhu, Shaojun Li, Qingting Wang, Qianqian Zhang, Xin Yan, Limin Chai, Pengtao Liu, Yuqian Chen, Manxiang Li

Date of Publication : 2018-09-01

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells

Author: Xiang Zhang, Fan Zhao, Jian-Feng Zhao, Hui-Ying Fu, Xiao-Jun Huang, Bo-Dong Lv

Date of Publication : 2017-11-22

NOGO-B promotes EMT in lung fibrosis via MMP14 mediates free TGF-beta1 formation

Author: Ye Xiong, Jing Zhang, Lingzhi Shi, Yunye Ning, Ying Zhu, Si Chen, Meng Yang, Jingyu Chen, Guo-Wu Zhou, Qiang Li

Date of Publication : 2017-08-16

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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