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> Products > Primary Antibodies > FEN-1 Antibody

FEN-1 Antibody

Overview Description
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Catalog No : C10585
  • Reactivity
    Human,Mouse,Rat
  • Applications
    WB,IHC-p,IF/ICC,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
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Details
  • Product Name
    FEN-1 Antibody
  • Description
    The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
  • Catalog No
    C10585
  • Reactivity
    Human, Mouse, Rat
  • Applications
    WB, IHC-p, IF/ICC, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    FEN1; RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1
  • Molecular Weight (Da)
    42593
  • Gene Name
    FEN1
  • Protein Name
    Flap endonuclease 1
  • Human Gene ID
    2237
  • Human Swiss Prot No.
    P39748
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human FEN1. AA range:86-135
  • Specificity
    FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from COLO205 cells, using FEN1 Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunofluorescence analysis of HeLa cells, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Application Data
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Application Data
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Application Data
Western Blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody diluted at 1:500
Application Data
Western Blot analysis of various cells using FEN-1 Polyclonal Antibody diluted at 1:500

Primary Antibodies

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