The Src (Phospho-Tyr418) Phospho Sandwich ELISA Kit allows for qualitative detection of Src (Phospho-Tyr418) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
Hek 293 cells were grown to 90% confluency and were stimulated with 20% Serum and incubated for 1 hr. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Src P-Tyr418 versus untreated Hek 293 lysates.