The GSK3 beta (Phospho-Ser9) Phospho Sandwich ELISA Kit allows for qualitative detection of GSK3 beta (Phospho-Ser9) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and were stimulated with EGF 200 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of GSK3 beta P-Ser9 versus untreated HeLa lysates.