Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
Catalog No
D12827
Reactivity
Human, Mouse, Rat
Applications
WB
Modification
Meythl Specific
Source
Polyclonal Mouse
Dilution
WB: 1:500-1000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
