cAMP responsive element binding protein 1(CREB1)Homo sapiens. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],
Catalog No
N0162
Reactivity
Human,Mouse,Rat,Monkey
Applications
WB,IHC-p,IF/ICC,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
Molecular Weight (Da)
Gene Name
CREB1
Protein Name
Cyclic AMP-responsive element-binding protein 1
Human Gene ID
1385
Human Swiss Prot No.
P16220
Immunogen
Purified recombinant fragment of human CREB-1 expressed in E. Coli.
Specificity
CREB-1 Monoclonal Antibody detects endogenous levels of CREB-1 protein.
Immunofluorescence analysis of Hela cells using CREB-1 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) with DAB staining using CREB-1 Monoclonal Antibody.
Western Blot analysis using CREB-1 Monoclonal Antibody against K562 (1). Jurkat (2). L1210 (3). HEK293 (4). A431 (5). HeLa (6). Cos7 (7). PC-12 (8). and NIH/3T3 (9) cell lysate.
