The Tfact Fra-2 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of Fra-2 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ Fra-2 DNA-Binding ELISA detects active Fra-2 in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by UV(100J/M2) and PMA (200nM) before harvest.