The Tfact ATF7 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of ATF7 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ ATF7 DNA-Binding ELISA detects active ATF7 in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2). The DNA-transcription factor complex is treated with the stablization buffer.