The SYK (Phospho-Tyr348) Phospho Sandwich ELISA Kit allows for qualitative detection of SYK (Phospho-Tyr348) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and were stimulated with EGF 200ng/mL and incubated for 30 mins. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Src P-Tyr418 versus untreated HeLa lysates.