The GR (Phospho-Ser226) Phospho Sandwich ELISA Kit allows for qualitative detection of GR (Phospho-Ser226) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and were stimulated with PMA 150 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of GR P-Ser226 versus untreated HeLa lysates.