The p47 phox (Phospho-Ser359) Phospho Sandwich ELISA Kit allows for qualitative detection of p47 phox (Phospho-Ser359) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and stimulated with 30 ng/mL TNF-α for 30 mins. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of p47 phox P-Ser359 versus untreated HeLa lysates.
