The Chk2 (Phospho-Thr68) Phospho Sandwich ELISA Kit allows for qualitative detection of Chk2 (Phospho-Thr68) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and were stimulated with PMA 150 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Chk2 P-Thr68 versus untreated HeLa lysates.