The NF-κB p65 (Phospho-Ser276) Phospho Sandwich ELISA Kit allows for qualitative detection of NF-κB p65 (Phospho-Ser276) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
Jurkat cells were grown to 90% confluency and were stimulated with U.V. 150 J/s and incubated for 1 hr. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of NF-kB p65 P-Ser276 versus untreated Jurkat lysates.