The NF-κB p65 (Phospho-Ser536) Phospho Sandwich ELISA Kit allows for qualitative detection of NF-κB p65 (Phospho-Ser536) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and were stimulated with EGF 200ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of NFkB P-536 versus untreated HeLa lysates.