The SP1 (Phospho-Thr739) Phospho Sandwich ELISA Kit allows for qualitative detection of SP1 (Phospho-Thr739) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
Hek 293 cells were grown to 90% confluency and were stimulated with 20% Serum and incubated for 1 hr. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of SP1 P-Thr739 versus untreated Hek 293 lysates.