The Follistatin (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Follistatin concentrations within any experimental sample including cell lysates, serum and plasma.
Human Follistatin recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Follistatin was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Follistatin. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Follistatin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Follistatin proteins within the range of 23-3000 pg/ml.