The IL-33 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant IL-33 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0137
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
IL-33
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
O95760
NCBI Gene ID
90865
Target Synonyms
Interleukin-33, Interleukin-1 family member 11, Nuclear factor from high endothelial venules, NF-HEV, IL-1F11
Human IL-33 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human IL-33 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for IL-33. Each known sample concentration was assayed in triplicate. The OmniKine™ Human IL-33 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-33 proteins within the range of 32-4000 pg/ml.