The MCP-1 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0147
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
MCP-1
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P13500
NCBI Gene ID
6347
Target Synonyms
C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, SCYA2
Human MCP-1 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human MCP-1 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for MCP-1. Each known sample concentration was assayed in triplicate. The OmniKine™ Human MCP-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MCP-1 proteins within the range of 16-1000 pg/ml.