The Midkine (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Midkine concentrations within any experimental sample including cell lysates, serum and plasma.
Human Midkine recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Midkine was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Midkine. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Midkine ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Midkine proteins within the range of 32-2000 pg/ml.
