The RANTES (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant RANTES concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0157
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
RANTES
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P13501
NCBI Gene ID
6352
Target Synonyms
C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, D17S136E, SCYA5
Human RANTES recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human RANTES was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for RANTES. Each known sample concentration was assayed in triplicate. The OmniKine™ Human RANTES ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human RANTES proteins within the range of 16-2000 pg/ml.