The TNF-alpha (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant TNF-alpha concentrations within any experimental sample including cell lysates, serum and plasma.
Human TNF-alpha recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human TNF-alpha was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for TNF-alpha. Each known sample concentration was assayed in triplicate. The OmniKine™ Human TNF-alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TNF-alpha proteins within the range of 16-2000 pg/ml.
